A glycosyltransferase-enriched reconstituted membrane system for the synthesis of branched O-linked glycans in vitro

►Concentration/reconstitution of Golgi glycosyltransferases into (endogenous)lipid vesicles. ►High enzyme activity compared to microsomes. ►Reconstituted glycosyltransferases having their catalytic domains in an extravesicular orientation. ►One-pot and multistep reactions for rapid synthesis of core2-based O-glycans.