Investigating the effects of L- to D-amino acid substitution and deamidation on the activity and membrane interactions of antimicrobial peptide anoplin

► D-anoplin has the same antibacterial activity as L-anoplin. ► Deamidated anoplin occupies smaller area in a monolayer. ► Anoplin preferentially interacts with anionic phospholipid and forms clusters in zwitterionic monolayers. ► Lytic activity of anoplin depends on the membrane lipid composition.