کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798103 | 1053295 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Computational analysis of mutations in the transmembrane region of Vpu from HIV-1
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Computational analysis of mutations in the transmembrane region of Vpu from HIV-1 Computational analysis of mutations in the transmembrane region of Vpu from HIV-1](/preview/png/10798103.png)
چکیده انگلیسی
Vpu is an 81 amino acid integral membrane protein encoded by HIV-1. Its α-helical transmembrane (TM) domain (residues â¼6-28) enhances virion release by oligomerizing into bundles and forming ion-conducting channels across the plasma membrane. Its cytoplasmic domain (residues â¼29-81) is also α-helical and binds to the transmembrane protein CD4, inducing its degradation. Mutations within the TM domain have been found to abrogate enhanced particle release from the infected cell (Tiganos et al. Virology (1998) 251 96-107). A series of computational models of monomeric, pentameric and hexameric Vpu1-31 mutants have been constructed, embedded in fully hydrated lipid bilayers and subjected to a 3 ns molecular dynamics (MD) simulation. None of the mutations has any destabilizing effect on the secondary and tertiary structure. One of the mutants, in which the position of a tryptophan residue within the TM domain is altered, is known not to induce CD4 degradation; an extended kinked model of this mutant has been generated (Vpu1-52IVW-k) and during subsequent MD simulations, the bend between the TM and a part of the cytoplasmic domain is found to unwind and a complex salt bridge involving Lys-37 is formed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1716, Issue 1, 1 October 2005, Pages 1-10
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1716, Issue 1, 1 October 2005, Pages 1-10
نویسندگان
Andrew Candler, Matthew Featherstone, Rehan Ali, Leslie Maloney, Anthony Watts, Wolfgang B. Fischer,