کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799365 | 1054319 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptional control of the human urothelial-specific gene, uroplakin Ia
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transcriptional control of the human urothelial-specific gene, uroplakin Ia Transcriptional control of the human urothelial-specific gene, uroplakin Ia](/preview/png/10799365.png)
چکیده انگلیسی
The transcriptional control elements of tissue-specific genes may be exploited in the design of therapeutic constructs for use in human gene therapy. The uroplakins are a family of four proteins which form the asymmetric unit membrane of the urothelium. We have cloned the human uroplakin Ia gene and defined its genomic structure and transcriptional start site. Using quantitative RT-PCR in an extended panel of normal tissues, we have demonstrated highly urothelial-specific expression of this gene. A Dual-Luciferase assay was used to assess the transcriptional activity of a variety of promoter fragments of the human uroplakin Ia gene. A highly specific promoter fragment (consisting of 2147 bp of 5â²-flanking sequence, intron 1 and the 5â² UTR) was identified which regulated urothelial-specific expression in vitro. The human uroplakin Ia promoter identified has potential use in future gene therapy strategies to restrict transgene expression to the urothelium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1729, Issue 2, 30 June 2005, Pages 126-134
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1729, Issue 2, 30 June 2005, Pages 126-134
نویسندگان
G.D. Hall, R.J. Weeks, J. Olsburgh, J. Southgate, M.A. Knowles, P.J. Selby, J.D. Chester,