A plant peptide: N-glycanase orthologue facilitates glycoprotein ER-associated degradation in yeast

► Plant PNGase orthologue (AtPNG1) acts as a PNGase in yeast, facilitating ERAD. ► A catalytic mutant of AtPNG1 stabilises glycosylated ERAD substrates. ► Non-glycosylated ERAD substrate is degraded in an AtPNG1-independent manner. ► Binding between mutant AtPNG1 and the N-glycans on the ERAD substrate is observed.