Microtubule assembly-derived by dimerization of TPPP/p25. Evaluation of thermodynamic parameters for multiple equilibrium system from ITC data

► Tubulin Polymerization Promoting Protein/p25 occurs in monomeric and dimeric forms. ► The binding of GTP shifts the equilibrium towards the oligomerization of the protein. ► Mathematical model was elaborated to characterize the multiple equilibrium system. ► The dimeric form has higher tubulin polymerization promoting potency. ► The dimer may play a role in the fine tuning of microtubule assembly in vivo.