Fibrillation of transferrin

► Association of transferrin dimers detected by GPC, MS and aggregates by DLS. ► Collisional cross sections of apo- and holo-transferrin determined by ion mobility-MS. ► AFM detects flattened transferrin on mica, aggregation into fibrils in second layer. ► Fibres formed by dimers on carbon surfaces are characterised by TEM. ► Possible relationship between transferrin aggregation, iron deposition, and diseases.