کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10801044 1054724 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of light and chloramphenicol stress on incorporation of nitrogen into cyanophycin in Synechocystis sp. strain PCC 6308
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effects of light and chloramphenicol stress on incorporation of nitrogen into cyanophycin in Synechocystis sp. strain PCC 6308
چکیده انگلیسی
1H NMR spectroscopy was used to compare the uptake of nitrogen into cyanobacterial cyanophycin from two sources: from the breakdown of intracellular proteins and amino acids, and directly from the external growth medium. Cells grown initially in medium containing 14N-nitrate were transferred to 15N-nitrate medium in the presence of chloramphenicol in both low (4 μE m−2 s−1) and normal (100 μE m−2 s−1) light, and in low light alone. Cyanophycin was separated from cells and analyzed by 1H NMR spectroscopy. Cyanophycin is synthesized both from 14N (degradation of cellular proteins) and from 15N in the medium, the latter at a faster rate and to a greater extent under all conditions. SDS-PAGE showed that cyanophycin synthesis takes place by addition of monomers to already synthesized polymer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1725, Issue 2, 15 September 2005, Pages 241-246
نویسندگان
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