کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10801061 1054725 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biologically active bisquaternary ammonium chlorides: physico-chemical properties of long chain amphiphiles and their evaluation as non-viral vectors for gene delivery
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Biologically active bisquaternary ammonium chlorides: physico-chemical properties of long chain amphiphiles and their evaluation as non-viral vectors for gene delivery
چکیده انگلیسی
The biological properties of bisquaternary ammonium salts, which are derivatives of N,N-bisdimethyl-1,2-ethanediamine (bis-CnBEC), of general formula /CnH2n+1OOCCH2(CH3)2N+CH2CH2N+(CH3)2CH2COOCnH2n+1/2Cl−, were investigated (n=10, 12, 14). The interaction with model membrane was studied by differential scanning calorimetry experiments, and the apparent adiabatic molar compressibility of their solution as a function of concentration was obtained by sound velocity measurements. Their biological activities were assayed by Electrophoresis Mobility Shift, MTT proliferation, and transient transfection. All the investigated compounds interact with the DNA and are able to transfect DNA, when they are coformulated with DOPE, with an efficiency significantly greater than that of a standard commercial transfection reagent. Bis-C14BEC is the only molecule able to deliver DNA inside the cells without a helper lipid, as shown by EGFP expression, albeit with a low efficiency in comparison with a standard commercial transfection reagent. This may be due to a slightly different interaction of bis-C14BEC from bis-C10BEC and bis-C12BEC with phospholipid bilayers. Bis-C10BEC and bis-C12BEC show a slight fluidising effect, while bis-C14BEC increases stability of both the gel and the rippled gel phases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1722, Issue 2, 11 March 2005, Pages 224-233
نویسندگان
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