کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801744 | 1055633 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel Drosophila mitochondrial carrier protein acts as a Mg2Â + exporter in fine-tuning mitochondrial Mg2Â + homeostasis
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کلمات کلیدی
Zn2 +IPTGRT-PCRNSDMg2 +Ca2 + - Ca2 +isopropyl-β-d-thiogalactopyranoside - ایزوپروپیل-ب-دی-تیوگالکتوپیرانوزیدICP-MS - طیفسنجی جرمی پلاسمای جفتشده القاییinductively coupled plasma mass spectrometry - طیفسنجی جرمی پلاسمای جفتشده القاییYeast - مخمرDrosophila melanogaster - مگس سرکه، مگس میوه calcium - کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The homeostasis of magnesium (Mg2Â +), an abundant divalent cation indispensable for many biological processes including mitochondrial functions, is underexplored. In yeast, the mitochondrial Mg2Â + homeostasis is accurately controlled through the combined effects of importers, Mrs2 and Lpe10, and an exporter, Mme1. However, little is known about this Mg2Â + homeostatic process in multicellular organisms. Here, we identified the first mitochondrial Mg2Â + transporter in Drosophila, the orthologue of yeast Mme1, dMme1, by homologous comparison and functional complementation. dMme1 can mediate the exportation of mitochondrial Mg2Â + when heterologously expressed in yeast. Altering the expression of dMme1, although only resulting in about a 10% change in mitochondrial Mg2Â + levels in either direction, led to a significant survival reduction in Drosophila. Furthermore, the reduced survival resulting from dMme1 expression changes could be completely rescued by feeding the dMME1-RNAi flies Mg2Â +-restricted food or the dMME1-over-expressing flies the Mg2Â +-supplemented diet. Our studies therefore identified the first Drosophila mitochondrial Mg2Â + exporter, which is involved in the precise control of mitochondrial Mg2Â + homeostasis to ensure an optimal state for survival.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 1, January 2016, Pages 30-39
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 1, January 2016, Pages 30-39
نویسندگان
Yixian Cui, Shanke Zhao, Xudong Wang, Bing Zhou,