کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801959 | 1055649 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells
ترجمه فارسی عنوان
اثر کلسیم خارج سلولی بر بیان رجکتالسین و قابلیت زنده ماندن سلول های پروتئینی انسانی نئوپلاستیک و غیر عضلانی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Extracellular calcium (Ca2Â +o) and its receptor, the Ca2Â +-sensing receptor (CaSR), play an important role in prostate physiology, and it has been shown that the deregulation of Ca2Â + homeostasis and the overexpression of CaSR are involved in prostate cancer (PCa). Regucalcin (RGN), a Ca2Â +-binding protein that plays a relevant role in intracellular Ca2Â + homeostasis, was identified as an under-expressed protein in human PCa. Moreover, RGN was associated with suppression of cell proliferation, suggesting that the loss of RGN may favor development and progression of PCa. This work aims to unveil the role of Ca2Â +o on RGN expression and viability of non-neoplastic (PNT1A) and neoplastic (LNCaP) prostate cell lines. It was demonstrated that Ca2Â +o up-regulates RGN expression in both cell lines, but important differences were found between cells for dose- and time-responses to Ca2Â +o treatment. It was also shown that high [Ca2Â +]o triggers different effects on cell proliferation of neoplastic and non-neoplastic PCa cells, which seems to be related with RGN expression levels. This suggests the involvement of RGN in the regulation of cell proliferation in response to Ca2Â +o treatment. Also, the effect of Ca2Â +o on CaSR expression seems to be dependent of RGN expression, which is strengthened by the fact that RGN-knockdown in PNT1A cells increases the CaSR expression, whereas transgenic rats overexpressing RGN exhibit low levels of CaSR. Overall, our results highlighted the importance of RGN as a regulatory protein in Ca2Â +-dependent signaling pathways and its deregulation of RGN expression by Ca2Â +o may contribute for onset and progression of PCa.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 10, Part A, October 2015, Pages 2621-2628
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 10, Part A, October 2015, Pages 2621-2628
نویسندگان
Cátia V. Vaz, Daniel B. Rodrigues, SÃlvia Socorro, Cláudio J. Maia,