کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802156 | 1055663 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MTOR-independent induction of autophagy in trabecular meshwork cells subjected to biaxial stretch
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کلمات کلیدی
GFPsequestosomem.o.iBeta tubulinBECN1RFPBAFA1CtsBCHX3-MAmTORATGiOp3-methyladenine - 3-متیل آدنینbafilomycin A1 - بافیلومایسین A1TUBB - توبCASA - خانهcycloheximide - سیکلوهایسیمیدtrabecular meshwork - شبکه مشبکIntraocular pressure - فشار داخل چشمmammalian target of rapamycin - هدف پستانداران رپامایسینGreen fluorescence protein - پروتئین فلورسانس سبزRed fluorescence protein - پروتئین فلورسانس قرمزmultiplicity of infection - چندین عفونتautophagy-related genes - ژن های مربوط به autophagyCathepsin B - کاتئفسین BChloroquine - کلروکین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The trabecular meshwork (TM) is part of a complex tissue that controls the exit of aqueous humor from the anterior chamber of the eye, and therefore helps maintaining intraocular pressure (IOP). Because of variations in IOP with changing pressure gradients and fluid movement, the TM and its contained cells undergo morphological deformations, resulting in distention and stretching. It is therefore essential for TM cells to continuously detect and respond to these mechanical forces and adapt their physiology to maintain proper cellular function and protect against mechanical injury. Here we demonstrate the activation of autophagy, a pro-survival pathway responsible for the degradation of long-lived proteins and organelles, in TM cells when subjected to biaxial static stretch (20% elongation), as well as in high-pressure perfused eyes (30 mm Hg). Morphological and biochemical markers for autophagy found in the stretched cells include elevated LC3-II levels, increased autophagic flux, and the presence of autophagic figures in electron micrographs. Furthermore, our results indicate that the stretch-induced autophagy in TM cells occurs in an MTOR- and BAG3-independent manner. We hypothesize that activation of autophagy is part of the physiological response that allows TM cells to cope and adapt to mechanical forces.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1843, Issue 6, June 2014, Pages 1054-1062
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1843, Issue 6, June 2014, Pages 1054-1062
نویسندگان
Kristine M. Porter, Nallathambi Jeyabalan, Paloma B. Liton,