HOCl causes necrotic cell death in human monocyte derived macrophages through calcium dependent calpain activation

► HOCl exposure in the uM range causes to human macrophages to undergo a calcium dependent necrotic cell death. ► HOCl causes the Ca+ to enter the cytoplasm via plasma membrane L- and T- type Ca+ channels and RyR channels from the ER. ► Blocking of Ca+ channels or addition of calpain inhibitors prevents mitochondrial potential loss and lysosome failure. ► HOCl cause HMDM cell death through destabilization of cytosolic calcium control.