Destabilization of fructose 1,6-bisphosphatase-Z-line interactions is a mechanism of glyconeogenesis down-regulation in vivo

► GSK-3 inhibition results in destabilization of FBPase-Z-line interaction in muscle. ► Disruption of FBPase-Z-line binding results in a fast inhibition of glyconeogenesis. ► This allows for preferential usage of glucose to insulin-induced glycogen synthesis. ► GSK-3 inhibition leads to translocation of FBPase to mitochondria in vivo.