Knockdown of insulin receptor substrate 1 reduces proliferation and downregulates Akt/mTOR and MAPK pathways in K562 cells

► The effect of IRS1 silencing, by shRNA-lentiviral, in K562 cells was evaluated. ► IRS1 silencing decreased cell proliferation and colony formation. ► Inhibition of IRS1 arrested cells in the G0/G1 phase of cell cycle in K562 cells. ► IRS1 silencing resulted down modulation of Akt, P70S6K and ERK1/2 activity. ► IRS1 silencing downregulates Akt/mTOR and MAPK signaling in BCR-ABL cells.