Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication

► Histone chaperone FACT has at least two distinct chromatin-binding modes during DNA replication. ► An initial rapid binding of FACT independent of DNA replication events and a latter binding initiating after origin licensing essential for DNA replication. ► Cdc6 can inhibit the latter binding of FACT. ► This biphasic behavior of FACT may thus be a critical basis in elucidating the cross-talk between DNA replication and chromatin-modifying factors.