کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803181 | 1055801 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Taurine suppresses platelet-derived growth factor (PDGF) BB-induced PDGF-β receptor phosphorylation by protein tyrosine phosphatase-mediated dephosphorylation in vascular smooth muscle cells
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کلمات کلیدی
PI3KERKPNPGESphorbol 12-myristate 13-acetatepNPPPMA - LDC هاMAPK - MAPKMAPK/ERK kinase - MAPK / ERK kinasep-Nitrophenol - p-nitrofenolp-nitrophenyl phosphate - p-نیترفنیل فسفاتphosphoinositide-3-kinase - فسفونیوییدید-3-کینازMEK - مجاهدین خلقmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In atherosclerosis, abnormal vascular smooth muscle cell (VSMC) proliferation plays an important role to form fibroproliferative lesions and platelet-derived growth factor (PDGF)-BB is one of the most potent chemoattractants and proliferative factors for VSMCs. Taurine, sulfur-containing β-amino acid, has been considered to prevent the development of atherosclerosis, although the molecular mechanism remains obscure. Previously, we demonstrated that taurine significantly suppressed PDGF-BB-induced cell proliferation, DNA synthesis, immediate-early gene expressions and extracellular signal-regulated kinase (ERK) 1/2 phosphorylation in VSMCs. The present study was aimed at elucidating the precise molecular mechanism of taurine in PDGF-BB signaling pathway. We showed that taurine significantly suppressed PDGF-BB-induced phosphorylation of PDGF-β receptor and activation of its downstream signaling molecules such as Ras, MAPK/ERK kinase (MEK)1/2 and Akt. Because taurine did not attenuate phorbol 12-myristate 13-acetate (PMA)-induced PDGF-β receptor-independent ERK1/2 phosphorylation, we further investigated the suppressive mechanism of taurine in PDGF-β receptor level. Although taurine did not directly affect PDGF receptor autophosphorylation in vitro, taurine promoted PDGF-β receptor dephosphorylation and restored PDGF-BB-induced suppression of protein tyrosine phosphatase (PTPase) activity. Taken together, we propose that taurine could prevent or delay the progression of atherosclerosis by PTPase-mediated suppression of PDGF-β receptor phosphorylation, and by decreasing the activation of its downstream signaling molecules in VSMCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1745, Issue 3, 30 September 2005, Pages 350-360
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1745, Issue 3, 30 September 2005, Pages 350-360
نویسندگان
Hitoshi Yoshimura, Yuko Nariai, Masaharu Terashima, Toshifumi Mitani, Yoshinori Tanigawa,