α-Crystallin protects human arginosuccinate lyase activity under freeze-thaw conditions

► Cold inactivated argininosuccinate lyase could be restored by manipulating the conditions of the freeze-thaw cycle. ► Slow freezing or thawing results in loss of activity. ► α-Crystallin can act as cryo-protectant for argininosuccinate lyase during the freeze-thaw process.