Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes)

► Two novel Neu3 sialidase genes, neu3a and neu3b, have been identified and characterized from Oryzias latipes. ► Both sialidase specifically recognize gangliosides as substrates. ► The properties of neu3a appear to have been highly conserved in molecular evolution.