Blocking αvβ3 integrin by a recombinant RGD disintegrin impairs VEGF signaling in endothelial cells

► RGD disintegrins block avb3 integrin-mediated signaling. ► These proteins inhibit angiogenesis and are under current clinical trials. ► DisBa-01 downregulates VEGF and VEGFR expression in endothelial cells. ► This RGD disintegrin inhibits MMP-2 activity. ► DisBa-01 increases MMP-9 activity which increases its anti-angiogenic effect.