A nonradioisotope chemiluminescent assay for evaluation of 2-deoxyglucose uptake in 3T3-L1 adipocytes. Effect of various carbonyls species on insulin action

► A nonradioisotope chemiluminescent assay to evaluate glucose uptake activity. ► Evaluation of dose-effect various inhibitors on 2DG uptake into 3T3-L1 cells. ► 4-HNE and acrolein severely impairs insulin signalling into adipocytes.