Inhibitory effect of the recombinant Phoneutria nigriventer Tx1 toxin on voltage-gated sodium channels

► rPnTx1 order of potency: rNaV1.2 > rNaV1.7 ≈ rNaV1.4 ≥ rNaV1.3 > mNaV1.6 ≥ hNaV1.8. ► The IC50 for NaV1.2 was 33.7 ± 2.9 nM. ► rPnTx1 was inactive on several arthropod NaV channel isoforms. ► Of great interest is rPnTx1 high selectivity toward Nav1.7 channels. ► rPnTx1 becomes a promising tool for the development of analgesics.