Human parvovirus B19 virus-like particles: In vitro assembly and stability

► Human parvovirus B19-VP2 protein was expressed in bacteria as inclusion bodies. ► VP2-inclusion bodies were dissolved and refolded into virus-like particles. ► Refolding was systematically studied by varying pH, ionic strength and temperature. ► HPB19 virus-like particles can be quantitatively assembled in vitro.