New insights into tau-microtubules interaction revealed by isothermal titration calorimetry

► First characterization of tau-tubulin binding by isothermal titration calorimetry. ► Same tau binding mode on microtubules and curved protofilaments. ► High and low affinity constants with tau:tubulin stoichiometry of 0.2 and 0.8. ► Model of longitudinal binding of tau along protofilaments.