Comprehensive inhibitor profiling of the Proteus mirabilis metalloprotease virulence factor ZapA (mirabilysin)

► First inhibitor profiling of the metalloprotease ZapA from Proteus mirabilis. ► Details important information regarding S1′ and S2′ binding preferences of ZapA. ► Inhibitors have been demonstrated to protect substrates from ZapA-mediated degradation. ► May ultimately contribute to elucidation of the role of ZapA during infection. ► May lead to development of an 'anti-virulence' approach to management of P. mirabilis infections.