کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815224 | 1058461 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
3-Iodothyronamine increases transient receptor potential melastatin channel 8 (TRPM8) activity in immortalized human corneal epithelial cells
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کلمات کلیدی
TRPVDEDTRPMintracellular Ca2 +AmtBHCEC - hcecHuman corneal epithelium - اپیتلیوم قرنیه انسانیDry eye disease - بیماری چشم خشکTAM - تامhuman corneal epithelial cells - سلول های اپیتلیال قرنیه انسانBCTC - صورتهای مالیtransient receptor potential melastatin - میلزین بالقوه گیرنده گذراtransient receptor potential vanilloid - پتانسیل ترانزیتی وینیلوئیدCaP - کلاه لبه دارCalcium - کلسیمCapsaicin - کپسایسین یا کاپسیسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 3-Iodothyronamine increases transient receptor potential melastatin channel 8 (TRPM8) activity in immortalized human corneal epithelial cells 3-Iodothyronamine increases transient receptor potential melastatin channel 8 (TRPM8) activity in immortalized human corneal epithelial cells](/preview/png/10815224.png)
چکیده انگلیسی
3-Iodothyronamine (3T1AM) is an endogenous thyroid hormone metabolite that interacts with the human trace amine-associated receptor 1 (hTAAR1), a G-protein-coupled receptor, to induce numerous physiological responses including dose-dependent body temperature lowering in rodents. 3T1AM also directly activates cold-sensitive transient receptor potential melastatin 8 (TRPM8) channels in human conjunctival epithelial cells (HCjEC) at constant temperature as well as reducing rises in IL-6 release induced by transient receptor potential vanilloid 1 (TRPV1) activation by capsaicin (CAP). Here, we describe that 3T1AM-induced TRPM8 activation suppresses through crosstalk TRPV1 activation in immortalized human corneal epithelial cells (HCEC). RT-PCR and immunofluorescent staining identified TRPM8 gene and protein expression. Increases in Ca2 + influx induced by the TRPM8 agonists either 3T1AM (0.1-10 μM), menthol (500 μM), icilin (15-60 μM) or temperature lowering (either < 17 °C or > 17 °C) were all blocked by 10-20 μM BCTC, a mixed TRPV1/TRPM8 antagonist. BCTC blocked 3T1AM-induced recombinant TRPM8 activation of Ca2 + transients in an osteosarcoma heterologous expression system. The effects of BCTC in HCEC were attributable to selective TRPM8 inhibition since whole-cell patch-clamp currents underlying Ca2 + rises induced by 20 μM CAP were BCTC insensitive. On the other hand, Ca2 + transients induced by activating TRPV1 with either CAP or a hyperosmolar medium were suppressed during exposure to either 1 μM 3T1AM or 15 μM icilin. All of these modulatory effects on intracellular Ca2 + regulation induced by the aforementioned agents were attributable to changes in underlying inward and outward current. Taken together, TRPM8 activation by 3T1AM markedly attenuates and even eliminates hyperosmolar and CAP induced TRPV1 activation through crosstalk.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 3, March 2016, Pages 136-147
Journal: Cellular Signalling - Volume 28, Issue 3, March 2016, Pages 136-147
نویسندگان
Alexander Lucius, Noushafarin Khajavi, Peter S. Reinach, Josef Köhrle, Priyavathi Dhandapani, Philipp Huimann, Nina Ljubojevic, Carsten Grötzinger, Stefan Mergler,