NEMO differentially regulates TCR and TNF-α induced NF-κB pathways and has an inhibitory role in TCR-induced NF-κB activation

► RNA silencing NEMO in Jurkat E6.1 cells enhanced TCR-induced NF-κB reporter activity and IL-2 production ► In human primary T cells, CD3/CD28-induced NF-κB activation showed a para-curve correlation with the dosage of siRNA targeting NEMO ► TNF-α induced NF-κB activation showed a negative correlation with the knockdown extent of NEMO ► CD3/CD28 costimulation promoted cytoskeleton translocation of NEMO and IKKβ and the stoichiometric ratio of NEMO to IKKβ continuously shifted from high to low in cytoskeleton along with stimulation till 60 min.