کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10820514 1060709 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Three forms of cyclin B transcripts in the ovary of the kuruma prawn Marsupenaeus japonicus: Their molecular characterizations and expression profiles during oogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Three forms of cyclin B transcripts in the ovary of the kuruma prawn Marsupenaeus japonicus: Their molecular characterizations and expression profiles during oogenesis
چکیده انگلیسی
Cyclin B is a well known regulatory factor that plays a crucial role in mitosis and meiosis. Although the existence of cyclin B has been reported to be universal in a wide variety of eukaryotic organisms, no molecular data are available on crustacean species. In this study, three forms of cyclin B transcripts were first identified and characterized in the ovary of the commercially important kuruma prawn Marsupenaeus japonicus. The three transcripts (2.4, 1.9 and 1.7 kb) shared the identical sequence, with variations only in the length of 3′ untranslated regions (UTRs), and coexisted in the ovary as demonstrated by Northern blot analysis. The sequences of 3′ UTRs indicated that the distinct length UTRs of the transcripts is attributed to an alternative usage of various polyadenylation signals in the 3′ UTR. The open reading frame of 1203 bp encoded a putative 401 amino acid peptide. The deduced amino acid sequence shared 45-50% identities with the known B-type cyclin in other animals. Quantitative real-time RT-PCR revealed that the short transcript (1.7 kb) was the most abundant among the three transcripts, followed by the long (2.4 kb) and medium (1.9 kb), and the three forms of the transcripts displayed various expression profiles during oogenesis. In situ hybridization showed that the short transcript commenced expressing in the ova as early as the oogonia stage and accumulated largely at the perinucleolus (PN) stage, whereas almost no expression was found for the medium and long transcripts at the oogonia stage and moderate signals were detected at the PN stage. The differential expression of the three forms of transcripts suggested that various transcripts might perform different roles during oogenesis of the kuruma prawn.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 141, Issue 2, June 2005, Pages 186-195
نویسندگان
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