کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10821760 | 1061514 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Physiology and mRNA expression in rainbow trout (Oncorhynchus mykiss) after long-term exposure to the new antifoulant medetomidine
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کلمات کلیدی
IGF-1CYP1ASGRERODHSIGSTLSImRNAqPCRcDNA - cDNAComplementary DNA - DNA تکمیلیmessenger RNA - RNA messengerGrowth - رشدMicroarray - ریزآرایهcytochrome P450 1a - سیتوکروم P450 1aLiver somatic index - شاخص سمی کبدAntifouling - ضد زنگinsulin-like growth factor 1 - فاکتور رشد مانند انسولین 1Rainbow trout - قزل آلای رنگین کمانFish - ماهیMedetomidine - مدومودینnanomolar - نانومولارspecific growth rate - نرخ رشد خاصGrowth hormone - هورمون رشدquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیglutathione-S-transferase - گلوتاتیون S-ترانسفرازglutathione reductase - گلوتاتیون ردوکتازBlood glucose - گلوکز خون یا قند خون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Medetomidine is under evaluation for use as an antifouling agent, and its effects on non-target aquatic organisms are therefore of interest. In this study, rainbow trout was exposed to low (0.5 and 5.0Â nM) concentrations of medetomidine for up to 54Â days. Recently we have reported on effects on paleness and melanophore aggregation of medetomidine in these fish. Here, specific growth rates were investigated together with a broad set of physiological parameters including plasma levels of growth hormone (GH), insulin-like growth factor-I (IGF-I) and leptin, glucose and haemoglobin (Hb), hematocrit (Ht), condition factor, liver and heart somatic indexes (LSI, HSI). Hepatic enzyme activities of CYP1A (EROD activity), glutathione S-transferases (GST) and glutathione reductase (GR) were also measured. Additionally, hepatic mRNA expression was analysed through microarray and quantitative PCR in fish sampled after 31Â days of exposure. Medetomidine at both concentrations significantly lowered blood glucose levels and the higher concentration significantly reduced the LSI. The mRNA expression analysis revealed few differentially expressed genes in the liver and the false discovery rate was high. Taken together, the results suggest that medetomidine at investigated concentrations could interfere with carbohydrate metabolism of exposed fish but without any clear consequences for growth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 154, Issue 3, September 2011, Pages 234-241
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 154, Issue 3, September 2011, Pages 234-241
نویسندگان
Anna Lennquist, Noomi Asker, Erik Kristiansson, Adam Brenthel, Björn Thrandur Björnsson, Peter Kling, Maria Hultman, D.G. Joakim Larsson, Lars Förlin,