Discrimination against major groove adducts by Y-family polymerases of the DinB subfamily

- Escherichia coli DinB and human pol κ discriminate against major groove modified DNA bases.
- Activity is lowest with DNA templates containing ɛdA compared to N6-furfuryl-dA.
- Dpo4 is more accommodating of these modified bases than DinB or pol κ.
- DinB R35A mutation eliminates discrimination against N6-furfuryl-dA.