کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10823676 | 1061922 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase β bind microtubules in situ and in vitro
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase β bind microtubules in situ and in vitro The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase β bind microtubules in situ and in vitro](/preview/png/10823676.png)
چکیده انگلیسی
8-Oxoguanine DNA glycosylase (OGG1), a major DNA repair enzyme in mammalian cells and a component of the base excision repair (BER) pathway, was recently shown to be associated with the microtubule network and the centriole at interphase and the spindle assembly at mitosis. In this study, we determined whether other participants in the BER pathway also bind microtubules in situ and in vitro. Purified recombinant human DNA polymerase β (DNA Pol β) and purified recombinant mNEIL2 were chemically conjugated to fluorochromes and photosensitive dyes and used in in situ localization and binding experiments. Results from in situ localization, microtubule co-precipitation and site-directed photochemical experiments showed that recombinant human DNA Pol β and recombinant mNEIL2 associated with microtubules in situ and in vitro in a manner similar to that shown earlier for another BER pathway component, OGG1. Observations reported in this study suggest that these BER pathway components are microtubule-associated proteins (MAPs) themselves or utilize yet to be identified MAPs to bind microtubules in order to regulate their intracellular trafficking and activities during the cell cycle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 4, Issue 4, 4 April 2005, Pages 419-431
Journal: DNA Repair - Volume 4, Issue 4, 4 April 2005, Pages 419-431
نویسندگان
Kimberly A. Conlon, Holly Miller, Thomas A. Rosenquist, Dmitry O. Zharkov, Miguel Berrios,