کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10824410 | 1062365 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A hemocyte-specific integrin required for hemocytic encapsulation in the tobacco hornworm, Manduca sexta
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کلمات کلیدی
PBSNonidet P-40NP-40MSBmAbDOCRT-PCRPAGEMonoclonal antibody - آنتی بادی مونوکلونالpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدInsect immunity - ایمنی حشرهInnate immunity - ایمنی ذاتیtriethanolamine - تری اتانولامینrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدSodium deoxycholate - سدیم دگزیکسولاتPhosphate buffered saline - فسفات بافر شورRace - مسابقهHemocyte - هموسیتreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسTEA - چایCell adhesion - چسبندگی سلولیEncapsulation - کپسوله سازی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش حشره شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Upon encountering an object recognized as foreign, insect hemocytes aggregate in multiple layers on the surfaces of the object in a process known as encapsulation. For encapsulation to occur, hemocytes must switch from their usual nonadherent state to an adherent state, presumably by regulating the activity of adhesion proteins. Although detailed knowledge exists regarding the adhesion receptors for cells of the mammalian immune system, comparable information on adhesion molecules of insect hemocytes and their function in immune responses is extremely limited. We report here the identification of an integrin present exclusively on the surface of hemocytes in the tobacco hornworm, Manduca sexta. Monoclonal antibodies MS13 and MS34, which bind to plasmatocytes and block encapsulation, were used for immunoaffinity chromatography to isolate their corresponding hemocyte antigen, which was revealed to be the same integrin β subunit. A cDNA for this M. sexta integrin β1 was cloned and characterized. Integrin-β1 mRNA was detected by Northern analysis in hemocytes and not in other tissues tested. MS13 and MS34 were demonstrated to bind to a recombinant fragment of integrin β1 consisting of the I-like domain, consistent with their blocking of a ligand-binding site and subsequent disruption of plasmatocyte adhesion. Injection of double stranded integrin-β1 RNA into larvae resulted in decreased integrin β1 expression in plasmatocytes and significantly suppressed encapsulation. These results indicate that activation of ligand-binding by the hemocyte-specific integrin plays a key role in stimulating plasmatocyte adhesion leading to encapsulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Insect Biochemistry and Molecular Biology - Volume 35, Issue 5, May 2005, Pages 369-380
Journal: Insect Biochemistry and Molecular Biology - Volume 35, Issue 5, May 2005, Pages 369-380
نویسندگان
David M. Levin, Lisha N. Breuer, Shufei Zhuang, Sheri A. Anderson, James B. Nardi, Michael R. Kanost,