کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10825069 | 1063335 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification of a synthetic oligonucleotide by anion exchange chromatography: Method optimisation and scale-up
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purification of a synthetic oligonucleotide by anion exchange chromatography: Method optimisation and scale-up Purification of a synthetic oligonucleotide by anion exchange chromatography: Method optimisation and scale-up](/preview/png/10825069.png)
چکیده انگلیسی
A single-step chromatographic method for purification of a synthetic 20-mer oligonucleotide is described. Method optimisation was conducted at laboratory scale where 30 mg crude sample was purified per run with a yield of 17 mg pure oligonucleotide. The protocol was scaled-up in steps to achieve 5-, 58- and a final 230-fold scale-up. At the final scale, 7.0 g of crude material was purified with a yield of 4.1 g product. The purity of the oligonucleotide was in all scales higher than 97%. The cycle time was 110 min, which corresponds to a purification capacity of about 90 g crude oligonucleotide material per 24 h.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 64, Issue 3, 30 September 2005, Pages 216-225
Journal: Journal of Biochemical and Biophysical Methods - Volume 64, Issue 3, 30 September 2005, Pages 216-225
نویسندگان
Jamil Shanagar,