کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10825161 1063350 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical impedance behavior of DNA biosensor based on colloidal Ag and bilayer two-dimensional sol-gel as matrices
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Electrochemical impedance behavior of DNA biosensor based on colloidal Ag and bilayer two-dimensional sol-gel as matrices
چکیده انگلیسی
A novel method for fabrication of DNA biosensors has been developed by means of self-assembling colloidal Ag (Ag) to a thiol-containing sol-gel network. The thiol groups of 3-mercaptopropyltrimethoxysilane (MPTS) serve as binding sites for the covalent attachment to gold electrode surface. Then the one-dimensional network of silane unites (1dMPTS) was combined together into a two-dimensional sol-gel network (2dMPTS) by dipping into aqueous NaOH. The second silane layer (B2dMPTS) was formed by immersing electrodes back into the MPTS solution overnight, and then the Ag nanoparticles were chemisorbed onto the thiol groups of the second silane layer. Finally, the mercapto oligonucleotide was self-assembled onto the surface via the Ag nanoparticles. The modified process was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). In addition, we utilized the impedance spectroscopy as a platform for DNA sensing assay. The factors influencing the performance of the resulting biosensor were studied in detail. The linear range of the biosensor was from 8.0×10−9 to 1.0×10−6 M with a detection limit of 4.0×10−9 M at 3σ. In addition, the experiment results indicate that oligonucleotide immobilized on this way exhibits a good sensitivity and selectivity, high stability and a long-term maintenance of bioactivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 62, Issue 2, 28 February 2005, Pages 163-174
نویسندگان
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