کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10825788 | 1064673 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Autoimmune animal models in the analysis of the CD47-SIRPα signaling pathway
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کلمات کلیدی
EAE2,4-dinitro-1-fluorobenzeneDNFBCIIDCsCFAIBDCHScomplete Freund’s adjuvant - adjuvant دوست کاملcDCs - cdc هاcollagen-induced arthritis - آرتروز ناشی از کلاژنexperimental autoimmune encephalomyelitis - آنسفالومیلیت خودایمنی تجربیimmunoglobulin - ایمونوگلوبولینInflammatory bowel disease - بیماریهای التهابی رودهContact hypersensitivity - تماس با افزایش حساسیتCNS - دستگاه عصبی مرکزیDendritic cells - سلول های دندریتیکCIA - سیاcentral nervous system - سیستم عصبی مرکزیbone marrow - مغز استخوان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Signal regulatory protein α (SIRPα), also known as SHPS-1/SIRPA, is an immunoglobulin superfamily protein that binds to the protein tyrosine phosphatases Shp1 and Shp2 through its cytoplasmic region and is predominantly expressed in dendritic cells and macrophages. CD47, a widely expressed transmembrane protein, is a ligand for SIRPα, with the two proteins constituting a cell-cell communication system. It was previously demonstrated that the CD47-SIRPα signaling pathway is important for prevention of clearance by splenic macrophages of red blood cells or platelets from the bloodstream. In addition, this signaling pathway is also implicated in homeostatic regulation of dendritic cells and development of autoimmunity. Here we describe the detailed protocols for methods that were used in our recent studies to study the role of the CD47-SIRPα signaling pathway in autoimmunity. We also demonstrate that hematopoietic SIRPα as well as nonhematopoietic CD47 are important for development of experimental autoimmune encephalomyelitis. Thus, we here strengthen the importance of experimental animal models as well as other methods for the study of molecular pathogenesis of autoimmunity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 65, Issue 2, 15 January 2014, Pages 254-259
Journal: Methods - Volume 65, Issue 2, 15 January 2014, Pages 254-259
نویسندگان
Yoji Murata, Yasuyuki Saito, Tetsuya Kaneko, Takenori Kotani, Yoriaki Kaneko, Hiroshi Ohnishi, Takashi Matozaki,