کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10825971 1064692 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stable isotope labeling of mammals (SILAM) for in vivo quantitative proteomic analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Stable isotope labeling of mammals (SILAM) for in vivo quantitative proteomic analysis
چکیده انگلیسی
Metabolic labeling of rodent proteins with 15N, a heavy stable isotope of nitrogen, provides an efficient way for relative quantitation of differentially expressed proteins. Here we describe a protocol for metabolic labeling of rats with an 15N-enriched spirulina diet. As a case study, we also demonstrate the application of 15N-enriched tissue as a common internal standard in quantitative analysis of differentially expressed proteins in neurodevelopment in rats at two different time points, postnatal day 1 and 45. We briefly discuss the bioinformatics tools, ProLucid and Census, which can easily be used in a sequential manner to identify and quantitate relative protein levels on a proteomic scale.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 61, Issue 3, 15 June 2013, Pages 260-268
نویسندگان
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