کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10825978 | 1064692 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Constrained selected reaction monitoring: Quantification of selected post-translational modifications and protein isoforms
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کلمات کلیدی
PTMNeurograninTGF-βS/N - S / Npost-translational modification - اصلاح post-translationalProtein isoform - ایزوفرم پروتئینTransforming Growth Factor Beta - تبدیل بتا فاکتور رشدImmunoprecipitation - تخریب ایمنیMass spectrometry - طیف سنجی جرمیSignal-to-noise ratio - نسبت سیگنال به نویزselected reaction monitoring - نظارت بر واکنش انتخاب شدهQuantification - کوانتومی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Selected reaction monitoring (SRM) is a mass spectrometry method that can target signature peptides to provide for the detection and quantitation of specific proteins in complex biological samples. When quantifying a protein, multiple peptides are generated using a specific protease such as trypsin, thereby allowing a choice of signature peptides with robust signals. In contrast, signature peptide selection can be constrained when the goal is to monitor a specific post-translational modification (PTM) or protein isoform, as the signature peptide must include the amino acid residue(s) of PTM attachment or sequence variation. This can force the selection of a signature peptide with a weak SRM response or one that is confounded by high background. In this article, we discuss steps that can be optimized to maximize peptide selection and assay performance of constrained SRM assays, including tuning instrument parameters, fragmenting product ions, using a different protease, and enriching the sample. Examples are provided for phosphorylated or citrullinated peptides and protein isoforms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 61, Issue 3, 15 June 2013, Pages 304-312
Journal: Methods - Volume 61, Issue 3, 15 June 2013, Pages 304-312
نویسندگان
Xiaoqian Liu, Zhicheng Jin, Richard O'Brien, Joan Bathon, Harry C. Dietz, Eric Grote, Jennifer E. Van Eyk,