کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10826089 1064710 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Building and searching tandem mass (MS/MS) spectral libraries for peptide identification in proteomics
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Building and searching tandem mass (MS/MS) spectral libraries for peptide identification in proteomics
چکیده انگلیسی
Spectral library searching is an emerging approach in peptide identifications from tandem mass spectra, a critical step in proteomic data analysis. In spectral library searching, a spectral library is first meticulously compiled from a large collection of previously observed peptide MS/MS spectra that are conclusively assigned to their corresponding amino acid sequence. An unknown spectrum is then identified by comparing it to all the candidates in the spectral library for the most similar match. This review discusses the basic principles of spectral library building and searching, describes its advantages and limitations, and provides a primer for researchers interested in adopting this new approach in their data analysis. It will also discuss the future outlook on the evolution and utility of spectral libraries in the field of proteomics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 54, Issue 4, August 2011, Pages 424-431
نویسندگان
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