کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10826118 1064718 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single-molecule FRET of protein-nucleic acid and protein-protein complexes: Surface passivation and immobilization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Single-molecule FRET of protein-nucleic acid and protein-protein complexes: Surface passivation and immobilization
چکیده انگلیسی
Single-molecule fluorescence spectroscopy reveals the real time dynamics that occur during biomolecular interactions that would otherwise be hidden by the ensemble average. It also removes the requirement to synchronize reactions, thus providing a very intuitive approach to study kinetics of biological systems. Surface immobilization is commonly used to increase observation times to the minute time scale, but it can be detrimental if the sample interacts non-specifically with the surface. Here, we review detailed protocols to prevent such interactions by passivating the surface or by trapping the molecules inside surface immobilized lipid vesicles. Finally, we discuss recent examples where these methods were applied to study the dynamics of important cellular processes at the single-molecule level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 52, Issue 2, October 2010, Pages 192-200
نویسندگان
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