Purification of replication factors using insect and mammalian cell expression systems

► Detailed protocols for purification of various assemblies of Mcm complexes using an insect cell expression system are described. ► A highly efficient expression system in human 293T cells for large-sized proteins and complexes is described. ► Detailed description of transfection with in-house reagents is provided. ► Procedures for purification of various replication factors with this system are described. ► Other uses of this expression system are described.