کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10826167 1064728 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tips and tricks for preparing lampbrush chromosome spreads from Xenopus tropicalis oocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Tips and tricks for preparing lampbrush chromosome spreads from Xenopus tropicalis oocytes
چکیده انگلیسی
Due to their large size and fine organization, lampbrush chromosomes (LBCs) of amphibian oocytes have been for decades one of the favorite tools of biologists for the analysis of transcriptional and post-transcriptional processes at the cytological level. The emergence of the diploid Xenopus tropicalis amphibian as a model organism for vertebrate developmental genetics and the accumulation of sequence data made available by its recent genomic sequencing, strongly revive the interest of LBCs as a powerful tool to study genes expressed during oogenesis. We describe here a detailed protocol for preparing LBCs from X. tropicalis oocyte and give practical advice to encourage a large number of researchers to become familiar with these chromosomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 51, Issue 1, May 2010, Pages 37-44
نویسندگان
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