Studying the effect of crowding and dehydration on DNA G-quadruplexes

► Methods to study the effects of crowding/dehydrating agents on DNA G-quadruplexes. ► Effect of crowding/dehydrating agents on quadruplex stability. ► Effect of crowding/dehydrating agents on quadruplex-duplex equilibria. ► Effect of crowding/dehydrating agents on G-quadruplex binding properties.