کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10826636 1064809 2005 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
True and false discovery in DNA microarray experiments: Transcriptome changes in the hippocampus of presenilin 1 mutant mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
True and false discovery in DNA microarray experiments: Transcriptome changes in the hippocampus of presenilin 1 mutant mice
چکیده انگلیسی
In transcriptome profiling experiments using DNA microarrays, it is critical to maximize putatively true data discovery while keeping the false discovery rate at acceptable levels. Using previously published and verified transcriptome datasets of mice with genetically altered PS1 physiology, we present a simple, robust, and system-specific assessment of type I and type II errors in two independent microarray experimental series. We provide evidence to suggest that for maximizing true discovery and minimizing false discovery, statistical criteria alone are inferior to statistical significance plus magnitude of change criteria. Furthermore, we found that, regardless of the exact criteria used for determining differential expression, different data extraction protocols give rise to different discovery and false discovery rates. In addition, a large proportion of expression differences were both dataset and analytical approach dependent. The data assessment methods presented and discussed in this manuscript can be easily carried out on any microarray dataset using basic spreadsheet functions as the only tool needed. Finally, we provide an in-depth analysis of the hippocampal transcriptome of ΔE9 hPS1 transgenic mice and mice with a conditional ablation of the PS1 gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 37, Issue 3, November 2005, Pages 261-273
نویسندگان
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