Combined extraction of acyl carnitines and 26:0 lysophosphatidylcholine from dried blood spots: Prospective newborn screening for X-linked adrenoleukodystrophy

► Our assay combines existing method for acyl carnitines analysis and 26:0 lyso-PC. ► Our data indicates that there is no significant effect on acyl carnitines levels. ► The method is highly sensitive. ► Our method can be applied as a screen for X-ALD and peroxisomal disorders.