کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10835696 | 1066331 | 2012 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence suggesting that ghrelin O-acyl transferase inhibitor acts at the hypothalamus to inhibit hypothalamo-pituitary-adrenocortical axis function in the rat
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کلمات کلیدی
radio-immuno assayRIAFCSACTHPOMCRT-PCRCRHqPCRFBSDMSO - DMSOQuantitative PCR - PCR کمیadrenocorticotropin - آدرنوکورتیکوتروپینaldosterone - آلدوسترون Octanoic acid - اسید اکتانیکEIA - اینGoat - بزDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوfetal calf serum - سرم گوساله جنینPVN - مالیات بر ارزش افزودهRat - موش صحراییHPA - میلی بار یا هکتوپاسکالparaventricular nucleus - هسته پروژسترویکcorticotrophin releasing hormone - هورمون آزاد کننده کورتیکوتروپینGrowth hormone - هورمون رشدhypothalamo-pituitary-adrenal - هیپوتالامو-هیپوفیز-آدرنالpolymerase chain reaction - واکنش زنجیره ای پلیمرازreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسPCR - واکنش زنجیرهٔ پلیمرازproopiomelanocortin - پروپیوملانوکورتینCell culture - کشت سلولCorticosterone - کورتیکوسترونGhrelin - گرلین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Production of n-octanoyl-modified ghrelin (GHREL), an active form of the peptide requires prohormone processing protease and GHREL O-acyltransferase (GOAT), as well as n-octanoic acid. Recently a selective GOAT antagonist (GO-CoA-Tat) was invented and this tool was used to study the possible role of endogenous GHREL in regulating HPA axis function in the rat. Administration of GOAT inhibitor (GOATi) resulted in a notable decrease in plasma ACTH, aldosterone and corticosterone concentrations at min 60 of experiment. Octanoic acid (OA) administration had no effect on levels of studied hormones. Plasma levels of unacylated and acylated GHREL remained unchanged for 60Â min after either GOATi or OA administration. Under experimental conditions applied, no significant changes were observed in the levels of GOAT mRNA in hypothalamus, pituitary, adrenal and stomach fundus. After GOATi injection hypothalamic CRH mRNA levels were elevated at 30Â min and pituitary POMC mRNA levels at 60Â min. Both GOATi and OA lowered basal, but not K+-stimulated CRH release by hypothalamic explants and had no effect on basal or CRH-stimulated ACTH release by pituitary slices. Neither GOATi nor OA affected corticosterone secretion by freshly isolated or cultured rat adrenocortical cells. Thus, results of our study suggest that in the rat endogenous GHREL exerts tonic stimulating effect on hypothalamic CRH release. This effect could be demonstrated by administering rats with selected inhibitor of ghrelin O-acyltransferase, the enzyme responsible for GHREL acylation, a process which is absolutely required for both GHSR-1a binding and its central endocrine activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 35, Issue 2, June 2012, Pages 149-159
Journal: Peptides - Volume 35, Issue 2, June 2012, Pages 149-159
نویسندگان
Marcin Rucinski, Agnieszka Ziolkowska, Marta Szyszka, Anna Hochol, Ludwik K. Malendowicz,