کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840136 | 1067570 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitric oxide and hydrogen peroxide in tomato resistance
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کلمات کلیدی
MBTHSAR2-deoxyriboseDAF-2TPVPB. cinereaNBTAPXTCACATTBA4,5-diaminofluorescein diacetate - 4،5-دی آمین فلوئورسین دی سکتهcPTIO - eptirOH• - OH •ROS - ROSHydrogen peroxide - آب اکسیژنهsuperoxide anion - آنیون سوپر اکسیدtrichloroacetic acid - اسید ترشکلراکتیکjasmonic acid - اسید جاسونیکSalicylic acid - اسید سالیسیلیکDOR - دردsystemic acquired resistance - سیستمیک به دست آورد مقاومتResistance - مقاومتsodium nitroprusside - نیتروپروساید سدیمNitric oxide - نیتریک اکسیدH2O2 - هیدروژن پراکسیدHypersensitive reaction - واکنش حساسascorbate peroxidase - پراکسیداز آسکورباتیpolyvinylpyrrolidone - پلی وینیل پیرولیدونSNP - چندریختی تک-نوکلئوتیدCatalase - کاتالازguaiacol peroxidase - گوایاکول پراکسیدازTomato - گوجه فرنگیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nitric oxide (NO) has been postulated to be required, together with reactive oxygen species (ROS), for activation of disease resistance reactions of plants to infection with a pathogen or elicitor treatment. However, biochemical mechanisms by which ROS and NO participate in these reactions are still under intensive study and controversial debate. We previously demonstrated that o-hydroxyethylorutin when applied on tomato leaves (Lycopersicon esculentum Mill. cv. “Perkoz”) restricted Botrytis cinerea infection development. In this research we investigated ROS and NO generation in tomato plants treated with o-hydroxyethylorutin, non-treated and infected ones. The NO content was enhanced or decreased in the studied plants by supplying them with NO generator-SNP or scavenger-cPTIO. NO detection was carried out using diaminofluorescein diacetate (DAF-DA) in conjunction with confocal laser scanning microscopy. The influence of elevated and decreased levels of NO on B. cinerea infection development and ROS generation was studied. The elevated NO concentration in tomato leaves strongly decreased hydrogen peroxide concentration without affecting other studied ROS (superoxide anion and hydroxyl radical) levels. H2O2 concentrations in NO-supplied leaves were low regardless of further treatment of tomato leaves with o-hydroxyethylorutin or inoculation with B. cinerea. The low H2O2 concentration coincided with quick and severe infection development in NO-supplied leaves. As activities of enzymes generating (SOD EC 1.15.1.1)) and removing (APX EC 1.11.1.11, CAT EC 1.11.1.6) H2O2 were unchanged in the studied plants, the decrease in H2O2 concentration was probably due to a direct NO-H2O2 interaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 6, June 2005, Pages 623-635
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 6, June 2005, Pages 623-635
نویسندگان
Urszula MaÅolepsza, Sylwia Różalska,