کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840262 | 1067616 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oxidation of the flavonoid eriodictyol by tyrosinase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Oxidation of the flavonoid eriodictyol by tyrosinase Oxidation of the flavonoid eriodictyol by tyrosinase](/preview/png/10840262.png)
چکیده انگلیسی
A pathway is proposed for the oxidation of the flavonoid eriodictyol by mushroom tyrosinase. In it, the enzymatic oxidation of eriodictyol leads to the formation of eriodictyol-o-quinone, which undergoes the nucleophilic attack of another eriodictyol unit to yield a dimer. This dimer is then oxidized by the eriodictyol-o-quinone. The reaction was followed by recording the time course of formation of this second o-quinone at 475Â nm. Progress curves at this wavelength showed the appearance of a lag, the length of which varied with enzyme and substrate concentrations, and which must have been caused by the chemical reactions taking place after the enzymatic reaction. When eriodictyol oxidation was studied in the presence of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH), which competes with the substrate in the reaction with eriodictyol-o-quinone, the lag disappeared. The kinetic parameters were similar with and without MBTH. Eriodictyol oxidation was inhibited by tropolone, which behaved as a slow-binding inhibitor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 9, September 2005, Pages 866-873
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 9, September 2005, Pages 866-873
نویسندگان
Mercedes Jiménez-Atiénzar, Josefa Escribano, Juana Cabanes, Fernando GandÃa-Herrero, Francisco GarcÃa-Carmona,