کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10840767 1067849 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation of peanut genes encoding arachins and conglutins by expressed sequence tags
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Isolation of peanut genes encoding arachins and conglutins by expressed sequence tags
چکیده انگلیسی
Seed storage proteins commonly comprise various groups of multiple isoforms encoded by different gene families. Arachin (11S globulin), conarachin (7S globulin) and conglutin (albumin) are the three major storage proteins in peanut (Arachis hypogaea L.). In order to isolate peanut seed storage protein genes, we sequenced and analyzed more than 400 randomly selected clones from a cDNA library derived from mid-maturation stage cotyledons of peanut. Our analysis indicated that arachin isoform precursors were encoded by at least five genes, whereas conglutins were encoded by more than six genes. The full-length cDNAs that encode isoforms of five arachin and six conglutin precursors were obtained. Among these cDNA clones, the deduced polypeptide of Ara 1 has about 99% similarity to that of peanut allergen Ara h 3. In addition, the deduced amino acid sequences of three conglutins, AhCONG 1, AhCONG 2 and AhCONG 5, are identical to those of peanut allergens Ara h 2.02, Ara h 2 and Ara h 6, respectively. Our study identified four new members (Ara 2 to Ara 5) of arachin family and two new members (AhCONG 3 and AhCONG 6) of conglutin family in peanut.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 169, Issue 2, August 2005, Pages 439-445
نویسندگان
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