کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10841827 | 1067902 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of transgenic plants resistant to leaf blast disease in finger millet (Eleusine coracana (L.) Gaertn.)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Production of transgenic plants resistant to leaf blast disease in finger millet (Eleusine coracana (L.) Gaertn.) Production of transgenic plants resistant to leaf blast disease in finger millet (Eleusine coracana (L.) Gaertn.)](/preview/png/10841827.png)
چکیده انگلیسی
Reproducible protocols for in vitro plant regeneration and genetic transformation have been established in finger millet using particle-inflow gun-mediated method. Using the optimized protocol, >4000 plantlets were regenerated from the callus of each shoot-tip explant within 75 days. Plasmid construct pPur, containing uid A gene driven by CaMV 35S promoter, was used for developing the transformation system. A gene coding for an antifungal protein (PIN) of prawn was chemically synthesized and was cloned into bacterial and plant expression vectors. Embryogenic calli were co-bombarded with the construct containing pin gene (pPin 35S) and another construct containing bar gene (pBar 35S) driven by CaMV 35S promoter. For stable transformation, the co-bombarded calli were cultured on phosphinothricin (PPT)-supplemented medium. In primary transformants, stable integration and expression of pin gene was confirmed by Southern and Northern blot analyses. Fungal bioassays and basta leaf dip test of T1 transformants disclosed monogenic 3 resistant:1 susceptible transgene segregations. Also, bar and pin genes showed co-segregation in the three T1 progenies analysed, implying single site integration of these genes. This study, first of its kind, reports the production of pin expressing transgenic finger millet exhibiting high-level resistance to leaf blast disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 169, Issue 4, October 2005, Pages 657-667
Journal: Plant Science - Volume 169, Issue 4, October 2005, Pages 657-667
نویسندگان
A. Madhavi Latha, K. Venkateswara Rao, V. Dashavantha Reddy,