کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10843130 1069184 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems
چکیده انگلیسی
Recombinant expression of membrane-bound cytochrome P450s in bacterial expression systems provide a well-established system capable of producing large yields of catalytically active protein. As the biochemical knowledge regarding cytochrome P450s increases, so does the efficiency of protein expression through various modifications that do not disrupt the functional properties of the protein of interest. Changes such as N-terminal modifications, reduction of secondary mRNA structure, bacterial codon usage, selection of vector and host strain, as well as varying external growth conditions all appear to influence protein expression. Several optimizations are often required for sufficient expression of cytochrome P450s at the desired cellular localization. This review aims to comprehensively summarize and update the significant advances made in membrane protein P450 expression in bacterial expression systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 92, Issue 1, November 2013, Pages 77-87
نویسندگان
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